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uc berkeley macrolab vectors 2b t  (Addgene inc)


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    Structured Review

    Addgene inc uc berkeley macrolab vectors 2b t
    Uc Berkeley Macrolab Vectors 2b T, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 89 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/uc+berkeley+macrolab+vectors+2b+t/pET+His6+TEV+LIC+cloning+vector+(2B-T)+(Plasmid+%2329666)/bio_rxiv__64898__2026__02__02__703251-183-19-24
    Average 94 stars, based on 89 article reviews
    uc berkeley macrolab vectors 2b t - by Bioz Stars, 2026-11
    94/100 stars

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    Construct:

    Article Title: Structural basis for SARS-CoV-2 Nucleocapsid protein recognition by single-domain antibodies
    Article Snippet: .. Briefly, constructs were amplified by PCR from the IDT 2019-nCoV N positive control plasmid (IDT cat. # 10006625; NCBI RefSeq YP_009724397) and inserted by ligation-independent cloning into UC Berkeley Macrolab vectors 2B-T (AmpR, N-terminal His 6 -fusion; Addgene #29666) or 2C-T (AmpR, N-terminal His 6 -MBP fusion; Addgene #29706). .. Plasmids were transformed into E. coli strain Rosetta 2(DE3) pLysS (Novagen), and grown in the presence of ampicillin and chloramphenicol to an OD 600 of 0.8 at 37°C, induced with 0.25 mM IPTG, then grown for a further 16 hours at 18°C prior to harvesting by centrifugation.

    Article Title: Structural Basis for SARS-CoV-2 Nucleocapsid Protein Recognition by Single-Domain Antibodies
    Article Snippet: .. Briefly, constructs were amplified by PCR from the IDT 2019-nCoV N positive control plasmid (IDT cat. # 10006625; NCBI RefSeq YP_009724397) and inserted by ligation-independent cloning into UC Berkeley Macrolab vectors 2B-T (AmpR, N-terminal His 6 -fusion; Addgene #29666) or 2C-T (AmpR, N-terminal His 6 -MBP fusion; Addgene #29706). .. Plasmids were transformed into E. coli strain Rosetta 2(DE3) pLysS (Novagen), and grown in the presence of ampicillin and chloramphenicol to an OD 600 of 0.8 at 37°C, induced with 0.25 mM IPTG, then grown for a further 16 hours at 18°C prior to harvesting by centrifugation.

    Article Title: Structural Basis for SARS-CoV-2 Nucleocapsid Protein Recognition by Single-Domain Antibodies.
    Article Snippet: .. Briefly, constructs were amplified by PCR from the IDT 2019- nCoV N positive control plasmid (IDT cat. # 10006625; NCBI Frontiers in Immunology | www.frontiersin.org 2 RefSeq YP_009724397) and inserted by ligation-independent cloning into UC Berkeley Macrolab vectors 2B-T (AmpR, N-terminal His6-fusion; Addgene #29666) or 2C-T (AmpR, N-terminal His6-MBP fusion; Addgene #29706). .. Plasmids were transformed into E. coli strain Rosetta 2(DE3) pLysS (Novagen), and grown in the presence of ampicillin and chloramphenicol to an OD600 of 0.8 at 37°C, induced with 0.25 mM IPTG, then grown for a further 16 hours at 18°C prior to harvesting by centrifugation.

    Amplification:

    Article Title: Structural basis for SARS-CoV-2 Nucleocapsid protein recognition by single-domain antibodies
    Article Snippet: .. Briefly, constructs were amplified by PCR from the IDT 2019-nCoV N positive control plasmid (IDT cat. # 10006625; NCBI RefSeq YP_009724397) and inserted by ligation-independent cloning into UC Berkeley Macrolab vectors 2B-T (AmpR, N-terminal His 6 -fusion; Addgene #29666) or 2C-T (AmpR, N-terminal His 6 -MBP fusion; Addgene #29706). .. Plasmids were transformed into E. coli strain Rosetta 2(DE3) pLysS (Novagen), and grown in the presence of ampicillin and chloramphenicol to an OD 600 of 0.8 at 37°C, induced with 0.25 mM IPTG, then grown for a further 16 hours at 18°C prior to harvesting by centrifugation.

    Article Title: Structural Basis for SARS-CoV-2 Nucleocapsid Protein Recognition by Single-Domain Antibodies
    Article Snippet: .. Briefly, constructs were amplified by PCR from the IDT 2019-nCoV N positive control plasmid (IDT cat. # 10006625; NCBI RefSeq YP_009724397) and inserted by ligation-independent cloning into UC Berkeley Macrolab vectors 2B-T (AmpR, N-terminal His 6 -fusion; Addgene #29666) or 2C-T (AmpR, N-terminal His 6 -MBP fusion; Addgene #29706). .. Plasmids were transformed into E. coli strain Rosetta 2(DE3) pLysS (Novagen), and grown in the presence of ampicillin and chloramphenicol to an OD 600 of 0.8 at 37°C, induced with 0.25 mM IPTG, then grown for a further 16 hours at 18°C prior to harvesting by centrifugation.

    Article Title: A DNA damage-activated kinase controls bacterial immune pathway expression
    Article Snippet: .. For protein expression, E. roggenkampii CapK (NCBI WP_001567866.1) and CapS (NCBI WP_001567865.1) were amplified by PCR and cloned into UC Berkeley Macrolab vectors 2B-T (Addgene #29666; encoding an N-terminal TEV protease-cleavable His 6 -tag), or 13S-A (Addgene #48323) for expression and purification. ..

    Article Title: Structural Basis for SARS-CoV-2 Nucleocapsid Protein Recognition by Single-Domain Antibodies.
    Article Snippet: .. Briefly, constructs were amplified by PCR from the IDT 2019- nCoV N positive control plasmid (IDT cat. # 10006625; NCBI Frontiers in Immunology | www.frontiersin.org 2 RefSeq YP_009724397) and inserted by ligation-independent cloning into UC Berkeley Macrolab vectors 2B-T (AmpR, N-terminal His6-fusion; Addgene #29666) or 2C-T (AmpR, N-terminal His6-MBP fusion; Addgene #29706). .. Plasmids were transformed into E. coli strain Rosetta 2(DE3) pLysS (Novagen), and grown in the presence of ampicillin and chloramphenicol to an OD600 of 0.8 at 37°C, induced with 0.25 mM IPTG, then grown for a further 16 hours at 18°C prior to harvesting by centrifugation.

    Polymerase Chain Reaction:

    Article Title: Structural basis for SARS-CoV-2 Nucleocapsid protein recognition by single-domain antibodies
    Article Snippet: .. Briefly, constructs were amplified by PCR from the IDT 2019-nCoV N positive control plasmid (IDT cat. # 10006625; NCBI RefSeq YP_009724397) and inserted by ligation-independent cloning into UC Berkeley Macrolab vectors 2B-T (AmpR, N-terminal His 6 -fusion; Addgene #29666) or 2C-T (AmpR, N-terminal His 6 -MBP fusion; Addgene #29706). .. Plasmids were transformed into E. coli strain Rosetta 2(DE3) pLysS (Novagen), and grown in the presence of ampicillin and chloramphenicol to an OD 600 of 0.8 at 37°C, induced with 0.25 mM IPTG, then grown for a further 16 hours at 18°C prior to harvesting by centrifugation.

    Article Title: Structural Basis for SARS-CoV-2 Nucleocapsid Protein Recognition by Single-Domain Antibodies
    Article Snippet: .. Briefly, constructs were amplified by PCR from the IDT 2019-nCoV N positive control plasmid (IDT cat. # 10006625; NCBI RefSeq YP_009724397) and inserted by ligation-independent cloning into UC Berkeley Macrolab vectors 2B-T (AmpR, N-terminal His 6 -fusion; Addgene #29666) or 2C-T (AmpR, N-terminal His 6 -MBP fusion; Addgene #29706). .. Plasmids were transformed into E. coli strain Rosetta 2(DE3) pLysS (Novagen), and grown in the presence of ampicillin and chloramphenicol to an OD 600 of 0.8 at 37°C, induced with 0.25 mM IPTG, then grown for a further 16 hours at 18°C prior to harvesting by centrifugation.

    Article Title: A DNA damage-activated kinase controls bacterial immune pathway expression
    Article Snippet: .. For protein expression, E. roggenkampii CapK (NCBI WP_001567866.1) and CapS (NCBI WP_001567865.1) were amplified by PCR and cloned into UC Berkeley Macrolab vectors 2B-T (Addgene #29666; encoding an N-terminal TEV protease-cleavable His 6 -tag), or 13S-A (Addgene #48323) for expression and purification. ..

    Article Title: Structural Basis for SARS-CoV-2 Nucleocapsid Protein Recognition by Single-Domain Antibodies.
    Article Snippet: .. Briefly, constructs were amplified by PCR from the IDT 2019- nCoV N positive control plasmid (IDT cat. # 10006625; NCBI Frontiers in Immunology | www.frontiersin.org 2 RefSeq YP_009724397) and inserted by ligation-independent cloning into UC Berkeley Macrolab vectors 2B-T (AmpR, N-terminal His6-fusion; Addgene #29666) or 2C-T (AmpR, N-terminal His6-MBP fusion; Addgene #29706). .. Plasmids were transformed into E. coli strain Rosetta 2(DE3) pLysS (Novagen), and grown in the presence of ampicillin and chloramphenicol to an OD600 of 0.8 at 37°C, induced with 0.25 mM IPTG, then grown for a further 16 hours at 18°C prior to harvesting by centrifugation.

    Positive Control:

    Article Title: Structural basis for SARS-CoV-2 Nucleocapsid protein recognition by single-domain antibodies
    Article Snippet: .. Briefly, constructs were amplified by PCR from the IDT 2019-nCoV N positive control plasmid (IDT cat. # 10006625; NCBI RefSeq YP_009724397) and inserted by ligation-independent cloning into UC Berkeley Macrolab vectors 2B-T (AmpR, N-terminal His 6 -fusion; Addgene #29666) or 2C-T (AmpR, N-terminal His 6 -MBP fusion; Addgene #29706). .. Plasmids were transformed into E. coli strain Rosetta 2(DE3) pLysS (Novagen), and grown in the presence of ampicillin and chloramphenicol to an OD 600 of 0.8 at 37°C, induced with 0.25 mM IPTG, then grown for a further 16 hours at 18°C prior to harvesting by centrifugation.

    Article Title: Structural Basis for SARS-CoV-2 Nucleocapsid Protein Recognition by Single-Domain Antibodies
    Article Snippet: .. Briefly, constructs were amplified by PCR from the IDT 2019-nCoV N positive control plasmid (IDT cat. # 10006625; NCBI RefSeq YP_009724397) and inserted by ligation-independent cloning into UC Berkeley Macrolab vectors 2B-T (AmpR, N-terminal His 6 -fusion; Addgene #29666) or 2C-T (AmpR, N-terminal His 6 -MBP fusion; Addgene #29706). .. Plasmids were transformed into E. coli strain Rosetta 2(DE3) pLysS (Novagen), and grown in the presence of ampicillin and chloramphenicol to an OD 600 of 0.8 at 37°C, induced with 0.25 mM IPTG, then grown for a further 16 hours at 18°C prior to harvesting by centrifugation.

    Article Title: Structural Basis for SARS-CoV-2 Nucleocapsid Protein Recognition by Single-Domain Antibodies.
    Article Snippet: .. Briefly, constructs were amplified by PCR from the IDT 2019- nCoV N positive control plasmid (IDT cat. # 10006625; NCBI Frontiers in Immunology | www.frontiersin.org 2 RefSeq YP_009724397) and inserted by ligation-independent cloning into UC Berkeley Macrolab vectors 2B-T (AmpR, N-terminal His6-fusion; Addgene #29666) or 2C-T (AmpR, N-terminal His6-MBP fusion; Addgene #29706). .. Plasmids were transformed into E. coli strain Rosetta 2(DE3) pLysS (Novagen), and grown in the presence of ampicillin and chloramphenicol to an OD600 of 0.8 at 37°C, induced with 0.25 mM IPTG, then grown for a further 16 hours at 18°C prior to harvesting by centrifugation.

    Plasmid Preparation:

    Article Title: Structural basis for SARS-CoV-2 Nucleocapsid protein recognition by single-domain antibodies
    Article Snippet: .. Briefly, constructs were amplified by PCR from the IDT 2019-nCoV N positive control plasmid (IDT cat. # 10006625; NCBI RefSeq YP_009724397) and inserted by ligation-independent cloning into UC Berkeley Macrolab vectors 2B-T (AmpR, N-terminal His 6 -fusion; Addgene #29666) or 2C-T (AmpR, N-terminal His 6 -MBP fusion; Addgene #29706). .. Plasmids were transformed into E. coli strain Rosetta 2(DE3) pLysS (Novagen), and grown in the presence of ampicillin and chloramphenicol to an OD 600 of 0.8 at 37°C, induced with 0.25 mM IPTG, then grown for a further 16 hours at 18°C prior to harvesting by centrifugation.

    Article Title: Structural Basis for SARS-CoV-2 Nucleocapsid Protein Recognition by Single-Domain Antibodies
    Article Snippet: .. Briefly, constructs were amplified by PCR from the IDT 2019-nCoV N positive control plasmid (IDT cat. # 10006625; NCBI RefSeq YP_009724397) and inserted by ligation-independent cloning into UC Berkeley Macrolab vectors 2B-T (AmpR, N-terminal His 6 -fusion; Addgene #29666) or 2C-T (AmpR, N-terminal His 6 -MBP fusion; Addgene #29706). .. Plasmids were transformed into E. coli strain Rosetta 2(DE3) pLysS (Novagen), and grown in the presence of ampicillin and chloramphenicol to an OD 600 of 0.8 at 37°C, induced with 0.25 mM IPTG, then grown for a further 16 hours at 18°C prior to harvesting by centrifugation.

    Article Title: Structural Basis for SARS-CoV-2 Nucleocapsid Protein Recognition by Single-Domain Antibodies.
    Article Snippet: .. Briefly, constructs were amplified by PCR from the IDT 2019- nCoV N positive control plasmid (IDT cat. # 10006625; NCBI Frontiers in Immunology | www.frontiersin.org 2 RefSeq YP_009724397) and inserted by ligation-independent cloning into UC Berkeley Macrolab vectors 2B-T (AmpR, N-terminal His6-fusion; Addgene #29666) or 2C-T (AmpR, N-terminal His6-MBP fusion; Addgene #29706). .. Plasmids were transformed into E. coli strain Rosetta 2(DE3) pLysS (Novagen), and grown in the presence of ampicillin and chloramphenicol to an OD600 of 0.8 at 37°C, induced with 0.25 mM IPTG, then grown for a further 16 hours at 18°C prior to harvesting by centrifugation.

    Ligation:

    Article Title: Structural basis for SARS-CoV-2 Nucleocapsid protein recognition by single-domain antibodies
    Article Snippet: .. Briefly, constructs were amplified by PCR from the IDT 2019-nCoV N positive control plasmid (IDT cat. # 10006625; NCBI RefSeq YP_009724397) and inserted by ligation-independent cloning into UC Berkeley Macrolab vectors 2B-T (AmpR, N-terminal His 6 -fusion; Addgene #29666) or 2C-T (AmpR, N-terminal His 6 -MBP fusion; Addgene #29706). .. Plasmids were transformed into E. coli strain Rosetta 2(DE3) pLysS (Novagen), and grown in the presence of ampicillin and chloramphenicol to an OD 600 of 0.8 at 37°C, induced with 0.25 mM IPTG, then grown for a further 16 hours at 18°C prior to harvesting by centrifugation.

    Article Title: Structural Basis for SARS-CoV-2 Nucleocapsid Protein Recognition by Single-Domain Antibodies
    Article Snippet: .. Briefly, constructs were amplified by PCR from the IDT 2019-nCoV N positive control plasmid (IDT cat. # 10006625; NCBI RefSeq YP_009724397) and inserted by ligation-independent cloning into UC Berkeley Macrolab vectors 2B-T (AmpR, N-terminal His 6 -fusion; Addgene #29666) or 2C-T (AmpR, N-terminal His 6 -MBP fusion; Addgene #29706). .. Plasmids were transformed into E. coli strain Rosetta 2(DE3) pLysS (Novagen), and grown in the presence of ampicillin and chloramphenicol to an OD 600 of 0.8 at 37°C, induced with 0.25 mM IPTG, then grown for a further 16 hours at 18°C prior to harvesting by centrifugation.

    Article Title: Structural Basis for SARS-CoV-2 Nucleocapsid Protein Recognition by Single-Domain Antibodies.
    Article Snippet: .. Briefly, constructs were amplified by PCR from the IDT 2019- nCoV N positive control plasmid (IDT cat. # 10006625; NCBI Frontiers in Immunology | www.frontiersin.org 2 RefSeq YP_009724397) and inserted by ligation-independent cloning into UC Berkeley Macrolab vectors 2B-T (AmpR, N-terminal His6-fusion; Addgene #29666) or 2C-T (AmpR, N-terminal His6-MBP fusion; Addgene #29706). .. Plasmids were transformed into E. coli strain Rosetta 2(DE3) pLysS (Novagen), and grown in the presence of ampicillin and chloramphenicol to an OD600 of 0.8 at 37°C, induced with 0.25 mM IPTG, then grown for a further 16 hours at 18°C prior to harvesting by centrifugation.

    Cloning:

    Article Title: Structural basis for SARS-CoV-2 Nucleocapsid protein recognition by single-domain antibodies
    Article Snippet: .. Briefly, constructs were amplified by PCR from the IDT 2019-nCoV N positive control plasmid (IDT cat. # 10006625; NCBI RefSeq YP_009724397) and inserted by ligation-independent cloning into UC Berkeley Macrolab vectors 2B-T (AmpR, N-terminal His 6 -fusion; Addgene #29666) or 2C-T (AmpR, N-terminal His 6 -MBP fusion; Addgene #29706). .. Plasmids were transformed into E. coli strain Rosetta 2(DE3) pLysS (Novagen), and grown in the presence of ampicillin and chloramphenicol to an OD 600 of 0.8 at 37°C, induced with 0.25 mM IPTG, then grown for a further 16 hours at 18°C prior to harvesting by centrifugation.

    Article Title: Structural Basis for SARS-CoV-2 Nucleocapsid Protein Recognition by Single-Domain Antibodies
    Article Snippet: .. Briefly, constructs were amplified by PCR from the IDT 2019-nCoV N positive control plasmid (IDT cat. # 10006625; NCBI RefSeq YP_009724397) and inserted by ligation-independent cloning into UC Berkeley Macrolab vectors 2B-T (AmpR, N-terminal His 6 -fusion; Addgene #29666) or 2C-T (AmpR, N-terminal His 6 -MBP fusion; Addgene #29706). .. Plasmids were transformed into E. coli strain Rosetta 2(DE3) pLysS (Novagen), and grown in the presence of ampicillin and chloramphenicol to an OD 600 of 0.8 at 37°C, induced with 0.25 mM IPTG, then grown for a further 16 hours at 18°C prior to harvesting by centrifugation.

    Article Title: Structural Basis for SARS-CoV-2 Nucleocapsid Protein Recognition by Single-Domain Antibodies.
    Article Snippet: .. Briefly, constructs were amplified by PCR from the IDT 2019- nCoV N positive control plasmid (IDT cat. # 10006625; NCBI Frontiers in Immunology | www.frontiersin.org 2 RefSeq YP_009724397) and inserted by ligation-independent cloning into UC Berkeley Macrolab vectors 2B-T (AmpR, N-terminal His6-fusion; Addgene #29666) or 2C-T (AmpR, N-terminal His6-MBP fusion; Addgene #29706). .. Plasmids were transformed into E. coli strain Rosetta 2(DE3) pLysS (Novagen), and grown in the presence of ampicillin and chloramphenicol to an OD600 of 0.8 at 37°C, induced with 0.25 mM IPTG, then grown for a further 16 hours at 18°C prior to harvesting by centrifugation.

    Expressing:

    Article Title: A DNA damage-activated kinase controls bacterial immune pathway expression
    Article Snippet: .. For protein expression, E. roggenkampii CapK (NCBI WP_001567866.1) and CapS (NCBI WP_001567865.1) were amplified by PCR and cloned into UC Berkeley Macrolab vectors 2B-T (Addgene #29666; encoding an N-terminal TEV protease-cleavable His 6 -tag), or 13S-A (Addgene #48323) for expression and purification. ..

    Clone Assay:

    Article Title: A DNA damage-activated kinase controls bacterial immune pathway expression
    Article Snippet: .. For protein expression, E. roggenkampii CapK (NCBI WP_001567866.1) and CapS (NCBI WP_001567865.1) were amplified by PCR and cloned into UC Berkeley Macrolab vectors 2B-T (Addgene #29666; encoding an N-terminal TEV protease-cleavable His 6 -tag), or 13S-A (Addgene #48323) for expression and purification. ..

    Purification:

    Article Title: A DNA damage-activated kinase controls bacterial immune pathway expression
    Article Snippet: .. For protein expression, E. roggenkampii CapK (NCBI WP_001567866.1) and CapS (NCBI WP_001567865.1) were amplified by PCR and cloned into UC Berkeley Macrolab vectors 2B-T (Addgene #29666; encoding an N-terminal TEV protease-cleavable His 6 -tag), or 13S-A (Addgene #48323) for expression and purification. ..



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